Coding

Part:BBa_K3807028:Design

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


neg control gRNA expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AtU6-26 ptomoter is an RNA Pol 3 promoter that is commonly used for RNA expression. Protospacer was designed not have a binding site in the BY-2 genome and the gRNA bosy was taken from the original pEvolvR plasmid

Source

Designed in silico using sequence from Addgene plasmid #113077 and synthesized by Twist

References

Halperin et al, 2018